Thursday, July 03, 2008

J. Am. Chem. Soc., 130 (27), 8856–8864, 2008.

Asymmetric Insertion of Membrane Proteins in Lipid Bilayers by Solid-State NMR Paramagnetic Relaxation Enhancement: A Cell-Penetrating Peptide Example

Yongchao Su, Rajeswari Mani, and Mei Hong*

A novel solid-state NMR technique for identifying the asymmetric insertion depths of membrane proteins in lipid bilayers is introduced. By applying Mn2+ ions on the outer but not the inner leaflet of lipid bilayers, the sidedness of protein residues in the lipid bilayer can be determined through paramagnetic relaxation enhancement (PRE) effects. Protein-free lipid membranes with one-side Mn2+-bound surfaces exhibit significant residual 31P and lipid headgroup 13C intensities, in contrast to two-side Mn2+-bound membranes, where lipid headgroup signals are mostly suppressed. Applying this method to a cell-penetrating peptide, penetratin, we found that at low peptide concentrations, penetratin is distributed in both leaflets of the bilayer, in contrast to the prediction of the electroporation model, which predicts that penetratin binds to only the outer lipid leaflet at low peptide concentrations to cause an electric field that drives subsequent peptide translocation. The invalidation of the electroporation model suggests an alternative mechanism for intracellular import of penetratin, which may involve guanidinium−phosphate complexation between the peptide and the lipids.

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