Solid-State NMR Spectroscopy Applied to a Chimeric Potassium Channel in Lipid Bilayers†
Robert Schneider, Christian Ader, Adam Lange, Karin Giller,Sönke Hornig, Olaf Pongs, Stefan Becker, and Marc Baldus*
We show that solid-state NMR can be used to investigate the structure and dynamics of a chimeric potassium channel, KcsA-Kv1.3, in lipid bilayers. Sequential resonance assignments were obtained using a combination of 15N−13C and 13C−13C correlation experiments conducted on fully labeled and reverse-labeled as well as C-terminally truncated samples. Comparison of our results with those from X-ray crystallography and solution-state NMR in micelles on the closely related KcsA K+ channel provides insight into the mechanism of ion channel selectivity and underlines the important role of the lipid environment for membrane protein structure and function.