CPL, M.E. Smith et al., 17O NMR; high-res

Enhancing resolution and sensitivity of 17O solid-state NMR through combining double rotation, 1H decoupling and satellite modulation for biomolecular applications

A.P. Howes, T. Anupõld, V. Lemaitre, A. Kukol, A. Watts, A. Samoson, M.E. Smith and R. Dupree

Department of Physics, University of Warwick, Coventry CV4 7AL, UK

Abstract

17O solid-state NMR is a highly sensitive probe of structural detail of organic solids but improvements in sensitivity and resolution are crucial for it to be applied to larger biological molecules. Here it is shown that high resolution (not, vert, similar1 ppm) and significant signal enhancement can be achieved by combining 1H decoupled double rotation (DOR), which narrows the lines by a factor of not, vert, similar100 compared to conventional magic angle spinning, and manipulation of the satellite transition populations to transfer magnetisation to the central transition, which produces a signal enhancement of not, vert, similar.