J. Am. Chem. Soc., 129 (47), 14717 -14723, 2007. 10.1021/ja0749690 S0002-7863(07)04969-4 Web Release Date: November 7, 2007 Copyright © 2007 American Chemical Society
Membrane-Bound Conformation of Peptaibols with Methyl-Deuterated -Amino Isobutyric Acids by 2H Magic Angle Spinning Solid-State NMR Spectroscopy
Kresten Bertelsen, Jan M. Pedersen, Brian S. Rasmussen, Troels Skrydstrup, Niels Chr. Nielsen, and Thomas Vosegaard*
Contribution from the Center for Insoluble Protein Structures (inSPIN), Interdisciplinary Nanoscience Center (iNANO) and Department of Chemistry, University of Aarhus, Langelandsgade 140, DK-8000 Aarhus C, Denmark firstname.lastname@example.org.
Received July 5, 2007
We present the use of 2H magic-angle spinning (MAS) NMR on methyl-deuterated -amino isobutyric acid (Aib) as a new method to obtain fast and accurate structural constraints on peptaibols in membrane-bound environments. Using nonoriented vesicle-reconstituted samples we avoid the delicate preparation of oriented samples, and the use of MAS ensures high sensitivity and thereby very fast acquisition of experimental spectra. Furthermore, given the high content (~40%) of Aib in peptaibols and the fact that the amino acid Aib may be synthesized from cheap starting materials, even in the case of 2H, 13C, or 15N labeling, this method is ideally suited for studies of the membrane-bound conformation of peptaibols.