Friday, February 03, 2006

JACS: CMAR sequence - Griffin

J. Am. Chem. Soc., ASAP Article 10.1021/ja0550430 S0002-7863(05)05043-2

Broadband Homonuclear Correlation Spectroscopy at High Magnetic Fields and MAS Frequencies

Gaël De Paëpe, Marvin J. Bayro, Józef Lewandowski, and Robert G. Griffin*

Department of Chemistry and Francis Bitter Magnet Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139


We present a new homonuclear recoupling sequence, CMAR, that allows observation of 2D 13C-13C correlation spectra at high magnetic fields and MAS frequencies (10-30 kHz). The main advantages of the sequence are that it provides efficient, broadband dipolar recoupling and concurrently decouples the 1H spins from the 13C's. Thus, no additional 1H decoupling is required during the mixing period, thereby significantly reducing the radio frequency power requirements for the experiment. Thus, CMAR significantly extends the range of applicability of the usual homonuclear recoupling techniques and should be of major interest for structure determinations of biomolecules at high magnetic fields.

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